Below is a picture of a Geiger Counter in our lab. The tube and counter are shown. On the counter, the user can set the counting time. The counter options include start, stop and reset. The display shows the number of radiation particles recorded during the counting time.
The virtual Geiger counter operates similar to the real one. Click here for the Virtual Geiger Counter. The Geiger counter has two sample holders. In each sample holder you can pick either an empty holder, 137Cs (2 μCi), or 54Mn (5 mCi). In the left holder you also have as an option the short lived isotope 137mBa as a sample. The detector has a dead time, and there is background radiation. The buttons are similar to a real Geiger counter. To operate: set the counting time and click the "start" button. Counting stops after the counting time. Then clear the counter with the "reset" button. To record counts from the 137mBa sample, you need to select the sample and click on "squeeze out Ba". Squeezing out the sample refreshes the Ba source, which has a short half life. The Ba source is only activated when it is in the sample holder and squeezed.
Some experiments that can be done:
Below is a picture of our NaI detector / Multi-Channel-Analyzer (MCA) setup. The complete setup includes: the NaI detector with photomultiplier tube, Power supply and amplifier box, and computer with MCA card.
For each gamma-detector experiment described below,
the MCA screen is displayed with different
sample options. Pick a sample from the list and
click on "Collect Data", which displays the spectrum. The total
number of channels is 1024, and the "pan" buttons
allows you to pan left or right the amounts indicated. There are two cursers.
The channel number and counts for each curser are displayed
underneath the screen.
Single Peak Fitting: The code allows the user to perform Gaussian peak fitting as follows: first set the left cursor (blue) with the "left cursor" button(s). Then set the right cursor using the "Width" button(s). Then click on "initial try" after Single Peak Fit. Click on "auto Fit" to improve the fit. Each time "auto Fit" is clicked a grid search is performed to minimize the total χ2. Keep clicking on "auto fit" until the total χ2 stops decreasing. The best-fit Gaussian parameters are displayed on the screen.
Double Peak Fitting: The code also allows for fitting two overlapping Gaussian peaks. Arrange the cursors so that they enclose both peaks. Then click on "initial try" after "Double Peak Fit". Two peaks should be displayed under the larger peak. Change the value in the box for "Center 2" to match the second peak. Then click on "manual Try". If the peaks are pretty close, then click on "auto fit" till the χ2 stops decreasing.
For information on the NaI detector experiments and the grid search, see "Data Analysis in the Undergraduate Nuclear Laboratory", Byron Curry, Dave Riggins, and P. B. Siegel, Am. J. Phys. 63, 71- 76 (January 1995).
Energy Calibration Experiment
To run the code, click on gamma detector (Calibration) You will see the MCA screen with 800 channels. You can pan to the right to see the last 200 channels. The samples include three standards and an unknown. The unknown is a single isotope which gives off 2 gamma's when it decays. Your goal is to determine the photopeak energies and the identity of the unknown.
The energy of the detected gamma is (approximately) proportional to the channel number. Use the standards listed below:
to determine the parameters of the linear relationship between channel number and energy. Then find the channel numbers of the photopeaks of the unknown, determine their energies from your calibration line, and interpolate to find the gamma energies of the unknown.
Measuring the activity of a Salt Substitute (KCl)
The Salt substitute was bought in a supermarket, and is pure KCl. To run the experiment, click on Salt Sample Activity You will see the MCA screen with 1024 channels. The relevant data on the calibration standards are listed in the table below:
5 years ago
All the calibration samples were recorded for two minutes counting time. Note that the calibration was done 5 years ago, so you need to account for the natural decay of the calibration standards. In addition to these calibration samples, the Salt Substitute sample and a background were counted for two hours. By background we mean that the detector was on for 2 hours with no sample present. All samples have approximately the same source-detector geometry. Your goal is to determine the activity in decays/sec of the Salt sample. An isotope of potassium, 40K, is radioactive and emits a gamma with an energy of 1460 KeV and a yield of 0.1068. One approach you can take is to first find the efficiency of the detector at the energies 662KeV, 511KeV, 1275KeV, 570KeV and 1063 KeV of the three standards. Make a graph of your results, and extrapolate to estimate the efficiency of the detector at 1460 KeV, which is the energy of the gamma emitted by K40. Using the counts under the 1460 KeV peak from the KCl sample, you can determine the activity of the sample. Remember to subtract the background 40K radiation, and to use the yield values in table above.
Attenuation of Gamma radiation in Lead Experiment
The program gamma attenuation in lead experiment contains gamma spectrum data with different absorbers between the source and detector. The source used was 137Cs, which gives off a gamma with energy 662 KeV and an x-ray with energy of 32 KeV. The 662 KeV photopeak is located near channel number 278. The data are taken with lead absorbers of various thickness to attenuate the gamma particles. The lead absorbers block the x-ray completely, but the 662 KeV gamma particles do pass through. All data were taken with the same source-detector geometry.
Your goal in the experiment is to see if the attenuation is exponential with absorber thickness, and if so, determine the linear mass attenuation coefficient for the 662 KeV gamma for lead . Measure the number of gamma particles that pass through the lead for the absorbers given. Use Gaussian curve fitting to determine the area under the photopeak.
The data are as follows:
|Sample||Lead Absorber Thickness (g/cm2)|
Collection time was two minutes in each case. Tables for the literature values of the attenuation coefficients can be found in tables.
Attenuation of X-ray radiation in Aluminum Experiment
The program x-ray attenuation in aluminum experiment contains spectrum data with different absorbers between the source and detector. The source used was 137Cs, which gives off a gamma with energy 662 KeV and an x-ray with energy of 32 KeV. The photopeak of the x-ray is around channel 90. The data are taken with aluminum absorbers of various thickness to attenuate the radiation. The aluminum absorbers do attenuate the 32 KeV x-ray. All data were taken with the same source-detector geometry.
Your goal in the experiment is to see if the attenuation is exponential with absorber thickness, and if so, determine the linear mass attenuation coefficient for the 32 KeV x-ray for aluminum. As with the previous experiment, measure the number of x-rays that pass through the aluminum for the absorbers given. Use Gaussian curve fitting to determine the area under the photopeak.
The data, for a two minute counting time, are as follows:
|Sample||Lead Absorber Thickness (g/cm2)|
Tables for the literature values of the attenuation coefficients can be found in tables. Note: the amplification has been doubled from the previous 662 KeV data, so the x-ray peak at around channel number 91 can be more clearly measured.
Below we show our high resolution Germanium detector from Canberra. The complete setup includes the detector with liquid nitrogen cooling, power supply, amplifier and a computer with a multi-channel analyzer card. There are 8192 channels.
The germanium detector is similar to the NaI detector, except that the resulution is significantly better and the data consist of 8192 channels. The computer program is similar to the NaI program. The screen displays 800 channels at a time. To move left or right through the complete spectrum, use the pan buttons as before. Gaussian peak fitting also works the same way as with the NaI detector program.
Environmental Sample Experiment
Click on Environmental Sample Experiment for the germanium detector for soil and rock analysis. There are three goals that you can have: (1) to determine the radioactive isotope content of the soil sample, (2) to determine the natural abundance of 235U in the rock sample, and (3) determine the age of the brazil nuts. Information about the gamma decay series for the natural occurring isotopes can be found in tables. Information about the characteristic x-rays given off by the various daughter isotopes can also be found in tables. These exercises may be difficult. We have written a program to assist in the analysis which you might find useful: soilfit.html To assist in the calculations, see "Gamma Spectroscopy of Environmental Samples", P.B. Siegel, Am. J. Phys. 81, 381-388 (May 2013).
Data for Ge detector:
1. Liquid Scintillation Detector is a program that simulates our liquid scintillation detector. You can choose four different samples: empty(none), 3H (tritium), 14C, or an "unknown". You can also choose different counting times. Both the 3H and the 14C samples had an activity of 100000 dpm when calibrated 17 years ago. Your goals are to:
2. RIA Calibration and RIA Experiment are two programs that simulate our radioimmunoassay experiments. Your goal in the experiment is to determine how the concentration of the hormone aldosterone changes in the blood of an animal over time after the injection of angiotensin II. The times after injection are 0, 2, 4, 6, and 8 hours. To carry out the experiment you need to first calibrate the assay technique with the program RIA Calibration. Once you have a calibration curve, you can run the program RIA Experiment to determine the aldosterone concentration over time.
3. Transport Assay Experiment is a program which simulates our Gaba transport assay experiment. Your goal is to determine the GABA transport rate as a function of the GABA concentration for both the control and the GAT3-expressing cell. The program allows you to input the "cold" Gaba concentration (from 0 to 500 micro-molar) added to the incubation solution. A pull down menu allows you to choose which sample is placed in the liquid scintillation detector: 10 micro-liters of solution, the control cell, or the GAT3-expressing cell. All incubation solutions contain the same amount of "hot" GABA. For more information on the experiment, see experiment 9 in our lecture notes: Radiation Biology Lecture Notes.
Physics Department| College of Science